Laboratory safety manual

Emergency information Major emergencies In the event of an accident in the laboratory which involves a fire, explosion, or a large release of a hazardous chemical: Evacuate the building by activating the nearest fire alarm. Call to give the details of the accident including the location, personal injuries, and types of hazardous material involved.

Laboratory safety manual

Army soldier, under the direction of Dr. Kaempf was tired of his MP duties at Detrick and was able to transfer to the sheet metal department working with the contractor, the H.

The meeting was to share knowledge and experiences regarding biosafetychemical, radiological, and industrial safety issues that were common to the operations at the three principal biological warfare BW laboratories of the U.

Beginning inthese conferences were planned to include non-classified sessions as well as classified sessions to enable broader sharing of biological safety information.

Emergency Procedures

It was not untilhowever, that conferences were held in a government installation not associated with a biological warfare program. By it began to include representatives from universities, private laboratories, hospitals, and industrial complexes.

Throughout the s, participation in the conferences continued to expand and by discussions began regarding the creation of a formal organization.

As ofABSA includes some 1, members in its professional association.

Laboratory safety manual

Levels[ edit ] Biosafety level 1[ edit ] Biosafety level 1 BSL-1 is suitable for work with well-characterized agents which do not cause disease in healthy humans. In general, these agents should pose minimal potential hazard to laboratory personnel and the environment.

Laboratory personnel must wash their hands upon entering and exiting the lab. Research with these agents may be performed on standard open laboratory benches without the use of special containment equipment. However, eating and drinking are generally prohibited in laboratory areas. Laboratory personnel have specific training in handling pathogenic agents and are directed by scientists with advanced training.

Access to the laboratory is limited when work is being conducted.


Extreme precautions are taken with contaminated sharp items. Certain procedures in which infectious aerosols or splashes may be created are conducted in biological safety cabinets or other physical containment equipment.

Biosafety level 3 is appropriate for work involving microbes which can cause serious and potentially lethal disease via the inhalation route.

All laboratory personnel are provided medical surveillance and offered relevant immunizations where available to reduce the risk of an accidental or unnoticed infection. This cannot be worn outside of the laboratory and must be discarded or decontaminated after each use.

The entrance to the laboratory must be separated from areas of the building with unrestricted traffic flow. Carpets are not permitted, and any seams in the floors, walls, and ceilings are sealed to allow for easy cleaning and decontamination.

These include Francisella tularensisMycobacterium tuberculosisChlamydia psittaciVenezuelan equine encephalitis virusEastern equine encephalitis virusSARS coronavirusCoxiella burnetiiRift Valley fever virusRickettsia rickettsiiseveral species of Brucellachikungunyayellow fever virusand West Nile virus.

Department of Microbiology - Clinical Laboratory Manual — Mount Sinai Hospital - Toronto

Additionally the cabinet and all materials within must be free of sharp edges in order to reduce the risk of damage to the gloves. Additionally airflow is tightly controlled to ensure that air always flows from "clean" areas of the lab to areas where work with infectious agents is being performed.

All laboratory waste, including filtered air, water, and trash must also be decontaminated before it can leave the facility. These include a number of viruses known to cause viral hemorrhagic fever such as Marburg virusEbola virusLassa virusCrimean-Congo hemorrhagic fever.

Additionally, poorly characterized pathogens which appear closely related to dangerous pathogens are often handled at this level until sufficient data are obtained either to confirm continued work at this level, or to work with them at a lower level.WHO Library Cataloguing-in-Publication Data Wo rld Health Organization.

Laboratory biosafety manual. – 3rd ed. webkandii.comnment of biohazards - methods webkandii.comtories - standards webkandii.comtory. The Laboratory Standard applies to all hazardous chemicals meeting the definition of "laboratory use" and having a potential for employee exposure.

Laboratory safety manual

The Laboratory Safety and Chemical Hygiene Plan (CHP) is a written program for ensuring the safe use of chemicals in laboratories at Indiana University. OHSAS Safety Manual - OHSAS The OHSAS Safety Manual for Occupational, health and safety management system (OHSAS) is addressing the requirements of OHSAS: The OHSAS is a standard of occupational, heaalth and safety management system which creates risk free environment in any organization.

(PDF version of this manual) Table of Contents Introduction Emergency Numbers Special Incident Reporting EHS Scope of Service Other Resources Chapter 1: Laboratory Safety at UNC-Chapel Hill Commitment to Safety The OSHA Laboratory Standard and the Chemical Hygiene Plan Cooperation .

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WHO | Laboratory Biosafety Manual - Third Edition